Reporter

Part:BBa_K762000:Design

Designed by: Menuka Samaranayake   Group: iGEM12_Wisconsin-Madison   (2012-09-25)

Translational Coupling Cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 592
    Illegal AgeI site found at 704
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

While creating this construct, there was a mystery sequence that appeared bewtween the Spe1 and Pst1 sites. If you cut with Spe1 and Pst1, this shouldn't be a problem. This is just a disclaimer for sequencing. Here is the sequence from the First Spe1 site through the Pst1 site.

ACTAGTAGCGGCCGCTACTAGTTCAGACCACGCTGATGCCCCCTGCAG

There is a mystery 27 base pairs between them, including an additional Spe1 site.

Source

References

Mendez-Perez, D., S. Gunasekaran, V. J. Orler, and B. F. Pfleger. "A Translation-coupling DNA Cassette for Monitoring Protein Translation in Escherichia Coli." Metabolic Engineering 4 (2012): 298-305.